Modified Telomeric Repeat Amplification
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Istvan Szatmari; Szilvia Tőkés; Christopher B. Dunn; Thomas J. Bardos; Janos Ara
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Article
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2000
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Elsevier Science
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English
⚖ 145 KB
A polymerase chain reaction (PCR)-based radioactive telomerase assay was developed in our laboratory which is quantitative and does not require electrophoretic evaluation (designated as TP-TRAP; it utilizes two reverse primers). The main steps of the assay include (1) extension of a 20-mer oligonucl