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A modified procedure for large-scale preparation of beef liver tRNA

โœ Scribed by Bonnie Robison; T.P. Zimmerman


Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
589 KB
Volume
37
Category
Article
ISSN
0003-2697

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A simple procedure for large-scale prepa
โœ M. Mukhopadhyay; N.C. Mandal ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 870 KB

A very simple, inexpensive procedure for preparing pure plasmid DNA from bacteria is described. In this method, lysozyme-induced spheroplasts are made in presence of 833 micrograms/ml of ethidium bromide which are then lysed by a mixture of Brij 58 and sodium deoxycholate, and the lysate is centrifu