๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A Modified Procedure for Fast Purification of T7 RNA Polymerase

โœ Scribed by Yong Li; Enduo Wang; Yinglai Wang


Book ID
115645504
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
72 KB
Volume
16
Category
Article
ISSN
1046-5928

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


Random mntagenesis of the gene for bacte
โœ Rechinsky, Vladimir O. ;Kostyuk, Dmitry A. ;Lyakhov, Dmitry L. ;Chernov, Boris K ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Springer ๐ŸŒ English โš– 421 KB

Random mutagenesis of the gene for bacteriophage T7 RNA polymerase was used to identify functionally essential amino acid residues of the enzyme. A two-plasmid system was developed that permits the straightforward isolation of T7 RNA polymerase mutants that had lost almost all catalytic activity. It