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A modified pET vector that augments heterologous protein expression

✍ Scribed by M. Walid Qoronfleh; Thau Ho

Publisher: Springer Netherlands
Year: 1993
Tongue: English
Weight: 281 KB
Volume: 15
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00128272

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✦ Synopsis

We describe a modification which can be carried out on the pET vector series that destroys the rop function in these vectors. The modified vectors have the following advantages : i) increased plasmid copy number, ii) augmented heterologous protein expression of the target gene, iii) elimination of plasmid incompatibility, and iv) they are smaller in size, consequently, they have higher transformation efficiency and can accommodate larger DNA inserts. Additionally, several restriction sites become unique allowing easier restriction mapping of inserts when required.

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