Flow cytometric analysis of chromosomes
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E. Severin; B. Ohnemus
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Article
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1982
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Springer
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English
β 572 KB
Chromosomes and interphase cells were harvested from cultures of the Chinese hamster line B14 F28 grown in medium containing BrdU up to four cell cycles and stained with the fluorescent dye 33342 Hoechst for flow cytometry. The newly synthetized BrdU-DNA is not stainable by the Hoechst dye which is