A Modified Method for the Indirect Quantitative Analysis of Phytate in Foodstuffs

✍ Scribed by G. Fruhbeck; R. Alonso; F. Marzo; S. Santidrian

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 572 KB
• Volume: 225
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1995.1145

✦ Synopsis

A simple, precise, and reproducible method to quantitatively assess total phytate content in foodstuffs has been developed. After (\mathrm{HCl}) extraction, samples were run through an anion-exchange column and total phytate content of the eluates was spectrophotometrically measured with the modified Wade reagent. The main changes introduced in our procedure refer to (\mathrm{pH}) adjustment in the extraction, dilution, and quantitation steps (pH 0.60, 6.00, and 3.00, respectively). Furthermore, the minimal extraction time for legumes ((2 \mathrm{~h})) was determined. In addition, the effect of protein content on phytate determination was tested. Phytate content directly measured in crude extracts omitting anion-exchange chromatographic purification was significantly lower ((P<0.01)) than values obtained using column separation, thus demonstrating that reliable phytate assessment in foodstuffs demands the utilization of anion-exchange chromatography. 1995 Academic Press, Inc.