A fluorometric method for the determinat
✍
P.A.George Fortes
📂
Article
📅
1986
🏛
Elsevier Science
🌐
English
⚖ 774 KB
A method to measure high-affinity binding sites for fluorescent ligands is described. The method is applied to the determination of (Na,K)-ATPase using the fluorescent ouabain derivative anthroylouabain (P. A. G. Fortes (1977) Biochemistry 16, 531-540). The method consists of measurements of the flu