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A modified Luria-Delbrück fluctuation assay for estimating and comparing mutation rates

✍ Scribed by Glenis J. Crane; Susan M. Thomas; Michael E. Jones

Book ID: 113194928
Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 904 KB
Volume: 354
Category: Article
ISSN: 0027-5107
DOI: 10.1016/0027-5107(96)00009-7

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Cells growing in culture are subject to mutation, and as mutation is a random event, the number of mutants in a culture will be a random variable. The size of the clone of mutants arising from a single mutational event depends on the timing of the mutation; the earlier the mutation the larger the co