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A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay

✍ Scribed by Lianwen Zhang; Feifei Ren; Jing Li; Xiaofeng Ma; Peng Wang

Book ID: 107667762
Publisher: BioMed Central
Year: 2009
Tongue: English
Weight: 231 KB
Volume: 11
Category: Article
ISSN: 1480-9222
DOI: 10.1007/s12575-009-9016-x

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## Abstract A new type of fluorescence assay for the determination of peroxidase (POx) activity is presented. The assay is based on the indication of the enzymatic consumption of H~2~O~2~ (HP), using a fluorescent europium–tetracycline (Eu~3~TC) complex as indicator. On addition of HP, this complex