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A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis

โœ Scribed by Chun Yi Liau; Chung-Sheng Lin


Book ID
115031954
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
217 KB
Volume
106
Category
Article
ISSN
1389-1723

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We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m