A modified bait and switch strategy for the generation of esterolytic abzymes using concerted catalytic mechanisms

✍ Scribed by Yoshiharu Iwabuchi; Shinwa Kurihara; Motoko Oda; Ikuo Fujii

• Book ID: 104261755
• Publisher: Elsevier Science
• Year: 1999
• Tongue: French
• Weight: 289 KB
• Volume: 40
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(99)00972-7

✦ Synopsis

Esterolytic abzymes were generated via immunization with a haptenic diphosphonate mounted with two distinct traps actuated by the "bait and switch" and "transition-state mimic" concepts. One monoclonal antibody, 12F12, was characterized in detail, and showed significant rate enhancement and unique substrate specificity. Experimental data support that the antibody catalyzes the reactions with a concerted catalytic mechanism.