A modified approach to identification of the sickle cell anemia mutation by means of allele-specific polymerase chain reaction

✍ Scribed by Klara R. Birikh; Oleg V. Plutalov; Eugene I. Schwartz; P. Sundari Devi; Yuri A. Berlin

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 276 KB
• Volume: 1
• Category: Article
• ISSN: 1059-7794
• DOI: 10.1002/humu.1380010511

✦ Synopsis

Communicated by Stylianos E. Antonarakis

The allele-specific PCR approach has been modified by introducing a second mismatch at the 3'penultimate link of the primer and used to identify the sickle cell anemia mutation (A + T transversion in the sixth codon of the human P-globin gene causing Glu + Val substitution in the protein), thus obviating the problem of an interpretationally ambiguous 3'-tetminal mismatch including T residue. o 1992 Wiley-Liss, Inc.