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A modified adsorption method for determining percentage of dead yeast cells

✍ Scribed by Marina L. R. Vairo


Publisher
John Wiley and Sons
Year
1962
Tongue
English
Weight
280 KB
Volume
4
Category
Article
ISSN
0006-3592

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✦ Synopsis


Abstract

The adsorption of methylene blue from different aqueous solutions by dead yeast cells follows Freundlich's law when the percentage of dead cells varies from 0 to 100. When the Fink and KΓΌhles methylene blue was used, there was observed a linear relation between the percentage of dead cells and the dye concentration at the equilibrium. The experimental errors were measured. A simpler and more precise colorimetric method for determining the percentage of dead yeast cells is deseribed.


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The rate of formation of spheroplasts of yeast can be used as an assay to study the structural integrity of cell walls. Lysis can be measured spectrophotometrically in hypotonic solution in the presence of Zymolyase, a mixture of cell wall-digesting enzymes. The optical density of the cell suspensio