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A moderately repeated DNA sequence of wheat and rye genomes

✍ Scribed by Marta Dobrzańska; Blanka Szurmak

Publisher
Springer
Year
1992
Tongue
English
Weight
271 KB
Volume
18
Category
Article
ISSN
0167-4412

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✦ Synopsis

A 371 base pair segment (bordered by Hind III and Eco RI cutting sites) of wheat embryo nuclear DNA has been cloned and sequenced. It is AT-rich (68 ~o), shares some sequence features with autonomously replicating sequence (ARS) elements, and occurs in approximately 7600 copies per haploid genome. When used as probe for blot hybridization to Hind III-digested wheat DNA, it gives an irregular series of hybridization bands. Essentially the same hybridization pattern was observed for rye DNA. It is concluded that this segment is distributed irregularly but, apparently, according to the same rule in both wheat and rye genomes.

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