A model system for the development of an optical biosensor based on lipid membranes and membrane-bound receptors

✍ Scribed by B. Klee; G.L. Duveneck; P. Oroszlan; M. Ehrat; H.M. Widmer

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 451 KB
• Volume: 29
• Category: Article
• ISSN: 0925-4005
• DOI: 10.1016/0925-4005(95)01698-8

✦ Synopsis

The reproducible and easy immobilization of receptors on sensor surfaces is a prerequisite for the development of receptor-based fibre optic biosensors. Using a fused silica fiber as the transducer, binding processes of luminescently labeled ligands can be monitored by evanescent wave sensor (EWS) technology. The vesicle fusion technique was chosen for the immobilization of membrane-bound receptors in order to preserve their binding specificity and activity, by embedding them in an environment similar to a lipid bilayer. The results of initial studies of repetitive cycles of lipid layer deposition and removal, indicating good reproducibility of lipid layer formation on the fiber, are presented. Using the binding of ftuorescently labeled streptavidin to a biotinylated lipid layer as a model system for receptor-ligand interaction, good sensitivity, combined with low non-specific binding were observed.