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A model of the mechanism of the separation of macromolecules in gel permeation chromatography on a packing with non-homogeneous pores

✍ Scribed by M. Kubín

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
802 KB
Volume
108
Category
Article
ISSN
1873-3778

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✦ Synopsis

In an attempt to resolve some controversies in published data, a theoretical model of the separation mechanism in gel permeation chromatography has been developed and tested. The model includes the effects of diffusion, both internal and longitudinal, and of steric exclusion; by using the concept of an impermeable boundary inside the particles of the packing, the model takes into account, in an elementary manner, the effect of the complicated geometrical form of pores. The dependences of the elution volume (characterized by the volume partition coefficient, Kopc) and of ,the height equivalent to a theoretical plate on relevant parameters of the model have been derived. The calculated dependence of the column efficiency on the elution volume is in good agreement with the experimental curves.

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