✦ LIBER ✦

A model for the action of cereal alpha amylases on amylose

✍ Scribed by Elizabeth A. MacGregor; Alex W. MacGregor

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 936 KB
Volume: 142
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(85)85025-4

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✦ Synopsis

A model is proposed to explain the action of cereal alpha amylases (EC 3.2.1.1) on such linear substrates as amylose. It is suggested that, at the active site of the enzyme, there are nine contiguous subsites, each capable of interacting with a glucose residue. An estimate is made of the energies involved in subsite-glucose interaction, and values obtained for the binding energies are used to predict the distributions of small oligosaccharides to be expected at intermediate stages of amylose hydrolysis catalyzed by cereal alpha amylases.

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