A Model for Copy Number Control of the Plasmid R1
β Scribed by M. Ehrenberg; A. Sverredal
- Book ID
- 115626368
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 573 KB
- Volume
- 246
- Category
- Article
- ISSN
- 0022-2836
No coin nor oath required. For personal study only.
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Two negatively acting functions - the CopA-RNA and the CopB protein - are involved in the control of replication of plasmid R1. They both act as inhibitors of expression of a gene, repA, which seems to be positively required for autonomous plasmid replication. Here we show that the two control funct
Site-specific deletions and insertions in the replication region of plasmid R1 have generated a new class of copy mutants that are present in the cell with 10-15-fold increased copy number. All mutations described inactivate a copy number control gene which is distinct from another cop inc gene that