A minimal human MBP Promoter-lacZ transgene is appropriately regulated in developing brain and after optic enucleation, but not in shiverer mutant mice
✍ Scribed by Wrabetz, Lawrence ;Taveggia, Carla ;Feltri, Maria Laura ;Quattrini, Angelo ;Awatramani, Raj ;Scherer, Steven S. ;Messing, Albee ;Kamholz, John
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 706 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0022-3034
No coin nor oath required. For personal study only.
✦ Synopsis
Previous studies, both in vitro and in that axons signal to this portion of the proximal MBP vivo, suggest that small portions of the mouse myelin promoter to fully activate MBP expression during mybasic protein (MBP) promoter are sufficient to activate regulated expression of MBP. To confirm our previous elinogenesis. Instead, in adult shiverer mice, another in vitro studies, we prepared transgenic mice with short setting in which MBP transcription is modulated, regions of the human MBP promoter fused to the lacZ transgene expression is not increased, in contrast to the reporter gene. We found that 750 nucleotides of the increased transcriptional activation of MBP previously proximal human MBP promoter is sufficient to activate reported in these mice. These data suggest that the oligodendrocyte-specific, developmentally regulated exregulatory region that mediates transcriptional activapression of lacZ in three independent lines. This protion of the MBP gene is modular, since discrete subremoter, however, does not activate expression of lacZ gions are required for activation in Schwann cells, durin Schwann cells in peripheral nerve or in adult mouse ing myelinogenesis in oligodendrocytes, during maintebrain. The relative levels of b-galactosidase specific acnance myelination in adult brain, and in the tivity, mRNA, and transcription parallel those of MBP dysmyelinating mutant shiverer mouse. ᭧ 1998 John mRNA during myelinogenesis. Thus, we exploited this