We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m
✦ LIBER ✦
A mild method of general use for covalent coupling of enzymes to chemically activated collagen films
✍ Scribed by Pierre R. Coulet; Jacques H. Julliard; Danièle C. Gautheron
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- English
- Weight
- 620 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0006-3592
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