A Convenient One-Step Extraction of Cell
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Nae-Cherng Yang; Wai-Meng Ho; Yu-Hsuan Chen; Miao-Lin Hu
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Article
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2002
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Elsevier Science
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English
β 58 KB
Cellular ATP is commonly determined as production of bioluminescence using a luciferin-luciferase reaction system. Before the measurement of bioluminescence, cellular ATP must first be extracted. Two commonly used extraction methods are: () Tris-borate buffer (pH 9.2) coupled with a heating process