A Microtiter Plate Assay for Screening Antioxidant Activity in Extracts of Marine Organisms
โ Scribed by Walter Dunlap; Lyndon Llewellyn; Jason Doyle; Yorihiro Yamamoto
- Publisher
- Springer
- Year
- 2003
- Tongue
- English
- Weight
- 204 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1053-6426
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A simple, inexpensive, and sensitive assay for peptidase activity has been devised. The assay was performed in a microtiter plate and was based on fluorogenic peptide substrates, many of which are commercially available. 7-Amino-4-methyl coumarin the fluorescent product liberated during an incubatio
The action of serine (and cysteine) proteases on peptide esters proceeds, as a generalization, orders of magnitude faster than the corresponding enzymatic hydrolysis of peptide bonds or peptide amides. Esterolysis liberates an alcohol while generating a free carboxyl group on the peptide; the proton