A continuous spectrophotometric method for assaying ATCase activity has been devised that couples the production of inorganic phosphate from the ATCase-catalyzed reaction to the phosphorolysis reaction catalyzed by purine nucleoside phosphorylase, using a chromophoric nucleotide analogue, methylthio
A microtiter plate assay for aspartate transcarbamylase
✍ Scribed by Anthony J. Else; Guy Hervé
- Book ID
- 107714825
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 317 KB
- Volume
- 186
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
cause no continuous assay for the detection of free phos-A new continuous coupled uv-spectrophotometric phate had been developed that is sensitive yet also conassay is described for two phosphate-releasing envenient and cost effective. Of the continuous assays zymes, aspartate transcarbamylase and A
We report the development of a microtiter plate assay for protein kinase C. Reaction components and enzyme samples (protein kinase C purified by phosphatidyIserine/cholesteroI affinity or DEAE-Sephacel ion-exchange chromatography) were added to wells of a 96-well microtiter plate. The assay was star