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A microtiter enzyme-linked immunosorbent assay for protein tyrosine phosphatase

✍ Scribed by Mishra, Shrikant; Hamburger, Anne W.

Book ID
121891950
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
819 KB
Volume
1157
Category
Article
ISSN
0304-4165

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Description of an enzyme-linked immunoso
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A solid immunoassay for the detection of protein tyrosine kinases has been developed. It is based on the binding of the synthetic polypeptide poly(Glu.Na,Tyr) 4:1 to microELISA wells, where the phosphorylation reaction takes place in the presence of ATP and enzyme. The phosphorylated tyrosine residu