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A microplate fluorimetric assay for the study of enzyme diversity in soils

โœ Scribed by M.-C Marx; M Wood; S.C Jarvis

Book ID
115547265
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
246 KB
Volume
33
Category
Article
ISSN
0038-0717

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โœ Harald T. Johansen; C.Graham Knight; Alan J. Barrett ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 89 KB

The cysteine endopeptidase legumain was recently discovered in mammalian cells, predominantly localized in the lysosomal system where it is believed to contribute to antigen processing for MHC class II. Here we describe rapid assay procedures for the enzyme in 96-well microplates with two substrates