A micromethod for the measurement of deuterium bound to carbon-6 of glucose to quantify gluconeogenesis in vivo

✍ Scribed by Satish C. Kalhan; Roopa Trivedi; Sheila Singh; Visvanathan Chandramouli; William C. Schumann; Bernard R. Landau

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 441 KB
• Volume: 30
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.1190301111

✦ Synopsis

Abstract

A method to measure the enrichment of deuterium in the hydrogens bound to C‐6 of glucose has been refined and taken to the micro‐scale. Glucose (≤0.2 mg) in small quantities of plasma samples is oxidized with sodium periodate. The formaldehyde formed contains the two hydrogens bound to C‐6 of glucose. The formaldehyde is condensed with ammonia to form hexamethylenetetramine (HMT), which can be directly determined by gas chromatography/mass spectrometry. All of the 12 hydrogens of HMT are from the formaldehyde and therefore deuterium enrichment as low as 0.1% on hydrogens bound to C‐6 of glucose can be measured with a coefficient of variation of less than 3%. The method can be used to quantify gluconeogenesis in vivo in humans by administering [^2^H~2~]O at an enrichment in total body water of only 0.5%, resulting in reproducibly measurable labeling of the hydrogens bound to C‐6 of glucose. The method is presented because of its potential value for the measurement of gluconeogenesis in humans in vivo at safe and acceptable doses of [^2^H~2~]O.