W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub
A micromethod for absorption of specific antibody using an enzyme-linked immunosorbent assay (ELISA)
โ Scribed by Vincent P. Calabrese
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 246 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0165-0270
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A 3-step enzyme-linked immunosorbent assay (ELISA) was developed for detecting IgA antibodies to purified Epstein-Barr virus (EBV) polypeptides. The 3-step procedure included the use of a mouse anti-human IgA monoclonal antibody (MAb) to amplify the IgA reaction. The 2 major EBV proteins used in thi
A direct enzyme-linked immunosorbent assay (ELISA) using species-specific monoclonal antibodies was developed for the detection and identification of Leishmania in sandflies. A titration of mock-infected Phlebotomus papatasi showed that fewer than 2000 L. major promastigotes could be detected. The p