A microassay for the determination of monoamine oxidase activity using electron capture gas chromatography

✍ Scribed by F.F. Farris; E.O. Magarian; F.O. Slininger

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 525 KB
• Volume: 88
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90449-9

✦ Synopsis

A sensitive and specific assay for determining monoamine oxidase (MAO) activity in serum and platelets is described. The procedure employs m-iodobenzylamine as substrate. The product, m-iodobenzaldehyde, is separated on an OV-17 column and measured by electron capture. Gas chromatography offers the advantage of analytical specificity without the need for additional procedural steps to eliminate potential interferences. Electron capture detection of the iodinated aldehyde is sufficiently sensitive to allow routine analysis on platelet samples of less than 15 pg of protein and serum aliquots of 50 ~1 or less. Analysis of approximately 80 samples per day may be accomplished by a single worker by employing an automatic sampling system for gas chromatographic injection. This fact, in addition to the small sample size, makes the method particularly suitable for the determination of large numbers of clinical samples.