A microassay for protein glycation based on the periodate method
โ Scribed by Nessar Ahmed; Anna J. Furth
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 257 KB
- Volume
- 192
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
The periodate assay for glycated protein has been adapted for use with a microplate reader. Up to 94 samples can be read in 40 s, and sensitivity has been improved so that only 2-40 nmol of protein-bound sugar are needed per well. Yields have been increased to over 90%, allowing in vitro glycation of human serum albumin to be assayed on 0.1-mg aliquots.
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