๐”– Bobbio Scriptorium
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A microassay for protein glycation based on the periodate method

โœ Scribed by Nessar Ahmed; Anna J. Furth


Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
257 KB
Volume
192
Category
Article
ISSN
0003-2697

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โœฆ Synopsis


The periodate assay for glycated protein has been adapted for use with a microplate reader. Up to 94 samples can be read in 40 s, and sensitivity has been improved so that only 2-40 nmol of protein-bound sugar are needed per well. Yields have been increased to over 90%, allowing in vitro glycation of human serum albumin to be assayed on 0.1-mg aliquots.


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