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A microassay for phallotoxins: Quantification of phallotoxins in Amanita species

✍ Scribed by Jerald E. Mullersman; James F. Preston

Book ID: 102985895
Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 565 KB
Volume: 119
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90584-x

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✦ Synopsis

The phallotoxin-mediated protection of bovine pancreatic deoxyribonuclease I from specific inhibition by rabbit muscle actin has been employed for the assay of pballotoxins. The use of this assay permits the quantification of as little as 6.3 ng of phalloidin. Phallotoxin concentrations are determined in crude extracts of carpophores from several Amanita species. On the basis of this assay, Amanita subafliacea contains as much as 8.1 mg phallotoxin/g dry weight. Amanita verna contains 0.02-0.03 mg/g dry weight. Levels of phallotoxin, which would have been undetectable by previous methods, are found in crude extracts of specimens of A. mutabilis and A. rubescens.

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