Various steps involved in the preparation of partially methylated alditol acetates (PMAAs) from glycoprotein-derived carbohydrates were improved to obtain the derivatives in a rapid manner with excellent yields. Carbohydrates were permethylated in dimethyl sulfoxide (DMSO), using a fine suspension o
A method of purification of partially methylated alditol acetates in the methylation analysis of glycoproteins and glycopeptides
β Scribed by Mark E. Lowe; Bo Nilsson
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 377 KB
- Volume
- 136
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
The structure of the methylated derivative (1) of the tetrasaccharide-alditol-O-beta-L-rhamnopyranosyl-(1----3)-O-beta-D- xylopyranosyl-(1----4)-O-beta-L-rhamnopyranosyl-(1----2)-1,5-di-O-acetyl -L- arabinitol has been determined solely on the basis of n.m.r. data.
A method is described for the analysis of positions of substitution of O-methyl and O-ethyl groups in commercial samples of O-methylcellulose and O-ethylcellulose, respectively. The method requires perethylation of O-methyl-cellulose and permethylation of O-ethylcellulose. Subsequent reductive cleav
Methylation analysis is still one of the most important methods for the structure analysis of oligosac~harides I. Gas chromatography-mass spe~tromet~, as well as 13C-and 'H-n.m.r. spectroscopy, is commonly used for identification of partially methylated monosaccharides. This requires numerous standa