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A method of estimating the amount of calcium bound to the metallochromic indicator arsenazo III

โœ Scribed by Tsuyoshi Ohnishi, S.


Book ID
123162392
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
724 KB
Volume
586
Category
Article
ISSN
0304-4165

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A spectrophotometric method to determine
โœ Lello Zolla; Maurizio Brunori ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 395 KB

The amount of CO bound to hemocyanin has been determined by titration with human hemoglobin (Hb). The spectrophotometric method makes use of the high athnity of Hb for CO, which (at pH 9) allows a complete transfer of the ligand from hemocyanin to hemoglobin. Taking advantage of the large spectral c