A method for the study of CO2exchanges in in vitro culturedVitis rupestrisplantlets

✍ Scribed by Matthieu Falque; Daniel Compan; Rose Galzy

• Book ID: 104619180
• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 436 KB
• Volume: 27
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00041287

✦ Synopsis

A CO 2 assay circuit adapted to in vitro culture was designed to investigate CO 2 exchanges in test tube-grown Vitis rupestris plantlets. The CO 2 concentration of the air in culture tubes was measured by injection of samples in the open circuit. It was observed under the culture conditions used that the CO 2 content stabilized during the light phase at 3 times the CO 2 compensation point.

Measurements of dark respiration under closed circuit conditions at every two-hour interval during the night did not reveal any limiting by lack of the substrate under mixotrophic culture conditions. A mathematical model of the influence of ambient CO 2 concentration on net CO 2 uptake rates under closed circuit conditions was devised and used to compare net photosynthesis at different lighting levels. Measurement of CO 2 evolution into C O S r e e air under open circuit conditions revealed a postillumination burst characteristic of photorespiration which increased with the temperature.