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A method for the purification of bacterial flagellin that allows simple upscaling

✍ Scribed by Yanina Hiriart; Agustina Errea; Dolores González Maciel; Juan Carlos Lopez; Martin Rumbo


Book ID
106565724
Publisher
Springer
Year
2011
Tongue
English
Weight
284 KB
Volume
28
Category
Article
ISSN
1573-0972

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We describe a straightforward and simple method for obtaining pure and active preparations of type 1 ribosome inactivating proteins (RIPs). The very high isoelectric point values, characteristic of these proteins, allow this purification in a single chromatographic step.