A method for the in vitro collection of pure gastric juice from isolated gastric mucosa

✍ Scribed by Edwards, Leslie E. ;Edwards, Carolyn T.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1951
• Tongue: English
• Weight: 695 KB
• Volume: 38
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030380205

✦ Synopsis

The study of isolated cells or tissues has certain advantages over studies using intact animals or whole organs. Conditions can be more completely control'led and indirect influences can be eliminated. However, the in vitro technique has the problem of maintaining the cells in a normal, physiological environment in which normal function can be maintained.

The technique to be described is a means by which isolated secretory gastric mucosa can be studied. Delrue, in 1930, and Gray et al. ('40) have shown that it is possible to obtain acid secretion from the isolated gastric mucosa of the frog. However, their methods did not permit collection o r measurement of the unmodified secretion but only the change in acid concentration in a salt solution to which the secretory cells were exposed. The present method (Edwards and Edwards, '48, '49) was designed to allow repeated collections of unmodified secretion. navies ('48) used sacks of mucosa that filled with secretion whicli could then be studied. Only one sample can be obtained by this method. Gas exchange studies can be done simultaneously w'th the seeretion. The sack method lias also been used by Davenport ('48) using mouse rriucosa.

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'Some preliminary designs of the apparatus were made in 1947 while one of the authors (L.E.) was working with the Fel's research group in the Philadelphia laboratories.

* The center hole in the celluloid rings exposes an area of 10 em2 of the mucosa.