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A method for the extraction of glycogen and metabolites from a single muscle sample

โœ Scribed by R. H. DALRYMPLE; R. HAMM


Book ID
108800085
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
348 KB
Volume
8
Category
Article
ISSN
0950-5423

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A procedure for extracting DNA, RNA, and protein from an L1210 leukemia cell sample is described. Sodium deoxycholate and 154 mM NaCl in the presence of phenol extracts cytoplasmic RNA. Sodium deoxycholate, paminosalicylate, and 1 M NaCl in the presence of phenol extracts the DNA. The DNA is then fr