𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A method for simultaneous determination of the two possible products of acetohydroxy acid synthase

✍ Scribed by Natan Gollop; Ze'ev Barak; David M. Chipman

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
690 KB
Volume
160
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A method for the simultaneous assay of 2-acetolactate and 2-aceto-2-hydroxybutyrate formation catalyzed by acetohydroxy acid synthase in the presence of its substrates pyruvate and 2-ketobutyrate is described. The method, appropriate for the study of the physiologically and mechanistically significant competition between the two reactions, involves oxidative decarboxylation ofthe acetohydroxy acids to the corresponding 2,3-diketones, transfer of the volatile diketones to methanol, and gas chromatographic analysis with electron-capture detection. Oxidative decarboxylation by air requires catalytic activation, and addition of iron salts is crucial to the success of the method with purified enzymes. 0 1987 Academic PESS, IIIC.

πŸ“œ SIMILAR VOLUMES

Two-rate method for simultaneous determi
Two-rate method for simultaneous determination of l-amino acid mixtures
✍ Yun-Sheng Hsieh; S.R. Crouch πŸ“‚ Article πŸ“… 1994 πŸ› Elsevier Science 🌐 English βš– 601 KB

A new kinetic-based method for simultaneous determination of binary mixtures is proposed that is simple, rapid, and minimizes errors arising from drift. This new method is based on the measurement of the rate at two points during the course of the enzymatic reaction and is therefore referred to as a

HPLC method for the simultaneous determi
HPLC method for the simultaneous determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid
✍ A. Gomez-Gomar; M. Gonzalez-Aubert; J. Costa-Segarra πŸ“‚ Article πŸ“… 1989 πŸ› Elsevier Science 🌐 English βš– 389 KB

A high-performance liquid chromatographic method suitable for the determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid is described. The column used is a Spherisorb-CN bonded phase at 25 degrees C. An aqueous solution of triethylamine (0.1%, v/v) at pH 2.5 is used as