A method for securing thymineless mutants from strains ofE. coli
β Scribed by Toshihiko Okada; Keiko Yanagisawa; F. J. Ryan
- Book ID
- 104996206
- Publisher
- Springer
- Year
- 1961
- Tongue
- English
- Weight
- 641 KB
- Volume
- 92
- Category
- Article
- ISSN
- 1617-4615
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A technique is presented by which mutations can be introduced into the Escherichia coli chromosome by gene replacement between the chromosome and a plasmid carrying the mutant gene. The segregational instability of plasmids in E. coli is used with high efficiency to isolate E. coli mutants. The meth
## Abstract Until now it has not been possible to obtain nuclear bodies from __Escherichia coli__ after treatment with rifampicin. It was generally assumed that the crossβconnections between the DNA double strands which are sensitive towards ribonuclease are destroyed under the influence of inhibit