A Method for Protein Assay in Laemmli Buffer

✍ Scribed by J.O. Karlsson; K. Ostwald; C. Kabjorn; M. Andersson

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 189 KB
• Volume: 219
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1994.1243

✦ Synopsis

A simple, cheap, and rapid assay for soluble and membrane-bound protein solubilized in Laemmli sodium dodecyl sulfate sample buffer is described. Following the addition of trichloroacetic acid to a final concentration of (24 %), the induced turbidity was measured at (570 \mathrm{~nm}) in a microplate reader, after an incubation period of approximately (10-30 \mathrm{~min}). The assay is suitable for measuring bovine serum albumin at a concentration of 15 to (500 \mu \mathrm{g} / \mathrm{ml}) in Laemmli buffer, corresponding to 2 to (75 \mu \mathrm{g}) protein per well on the microtiter plate. Other nonproteinaceous macromolecules in the sample may introduce an error. c 1994 Academin Press, Inc.