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A method for partitioning neutral detergent-soluble carbohydrates

โœ Scribed by Hall, Mary B; Hoover, William H; Jennings, Jocelyn P; Webster, Tammy K Miller

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
184 KB
Volume
79
Category
Article
ISSN
0022-5142

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โœฆ Synopsis

A method was developed to fractionate the neutral detergent-soluble carbohydrates (NDSC) in feedstuffs. Differential solubilities of carbohydrates in 80:20 (v/v) ethanol/water were used to partition NDSC into organic acids (OA) and mono-and oligosaccharides soluble in ethanol/water from starch and neutral detergent-soluble ยฎbre (NDSF) which are insoluble. Mono-and oligosaccharides (total ethanol/water-soluble carbohydrate) were measured on the ethanol/water extract, and starch was measured on the ethanol/water-insoluble residue. The OA and NDSF, the two most compositionally diverse NDSC fractions, were estimated by difference. The method allows partitioning of the NDSC on a nutritionally relevant basis into (1) organic acids, (2) total ethanol/water-soluble carbohydrate, (3) starch and (4) neutral detergent-soluble ยฎbre. The methods involved in this fractionation are relatively simple or are commonly used.

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