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A method for measuring sarcoplasmic reticulum calcium uptake in skeletal muscle using Fura-2

โœ Scribed by S.A. Warmington; M. Hargreaves; D.A. Williams

Book ID
114117962
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
989 KB
Volume
20
Category
Article
ISSN
0143-4160

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A Continuous Spectrophotometric Assay fo
A Continuous Spectrophotometric Assay for Simultaneous Measurement of Calcium Uptake and ATP Hydrolysis in Sarcoplasmic Reticulum
โœ B.S. Karon; E.R. Nissen; J. Voss; D.D. Thomas ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 504 KB

A continuous, spectrophotometric assay to simultaneously measure Ca uptake and ATP hydrolysis has been developed, in order to assess the function of the Ca-ATPase in skeletal and cardiac sarcoplasmic reticulum (SR) vesicles. The absorbance of Fura Red was measured continuously at \(490 \mathrm{~nm}\