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A method for measuring anion transfer across membranes of hemoglobin-free cells and vesicles by continuous monitoring of fluorescence

โœ Scribed by A. Darmon; O. Eidelman; Z.I. Cabantchik


Book ID
118308737
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
759 KB
Volume
119
Category
Article
ISSN
0003-2697

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A method for measuring anion transfer ac
โœ O. Eidelman; Z.I. Cabantchik ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 509 KB

A fluorometric method for continuous monitoring of anion transport across red blood cell membranes was developed. The method was based on the use of a fluorescent substrate, N-(2-aminoethylsulfonate)-7-nitrobenz-2-oxa-3-diazole) (NBD-taurine), whose intracellular fluorescence was quenched by hemoglo