A method for isolation of nuclei containing undegraded RNA from RNAase-rich plasmodia of Physarum polycephalum
β Scribed by Klaus-Dieter Nothacker; Armin Hildebrandt
- Book ID
- 119119722
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 647 KB
- Volume
- 950
- Category
- Article
- ISSN
- 0167-4781
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A simple technique is described for fractionating cells ofxenopus laevis using Triton X-100 and citric acid. Cells are treated for 1 min with a hypotonic buffer containing 0.5% Triton X-100 and RNAase inhibitors. The cells are then disrupted by adding citric acid to a final concentration of 1% for 2