A method for isolation of milled-wood lignin involving solvent swelling prior to enzyme treatment
✍ Scribed by J. -Y. Chen; Yuichi Shimizu; Mitsuo Takai; Jusuke Hayashi
- Book ID
- 104661478
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 490 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0043-7719
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✦ Synopsis
A new lignin isolation method has been developed. Wood and pulp were subjected to ball milling, swelled in an organic solvent, and then treated with a cellulase. The enzyme digestion time could be shortened to 1 day with this method. The lignin obtained has been named Swelled Enzyme Lignin (SEL). Swelling and enzyme digestion conditions and their effects on lignins were investigated. The SEL's from wood could be directly washed with water, while those from pulp had to be washed with aqueous acetic acid because they were water soluble. The purification of crude SEL's was accomplished by extracting them with dioxane-water, and then precipitating and washing with ethyl ether. Lignin yields were 24-67% based on the total amount of lignin present. The characteristics of the SEL's were further investigated by gel-permeation chromatography (GPC), infrared and 13C nuclear magnetic resonance (NMR) spectroscopy.
Symbols
The symbols used were the following: SUKP Softwood Unbleached Kraft Pulp BUKP Birch Unbleached Kraft Pulp TD Todomatsu (Abies sachalinensis) wood MWL Milled Wood Lignin MWLS (BK, TD) Milled Wood Lignin of SUKP (BUKP, TD) SEL-C Swelled Enzyme Lignin (crude) SELS-C SEL-C of SUKP, other materials named in the same way SELS-90 SEL-C of SUKP and extracted with 90% dioxane-water SELS-96 SEL-C of SUKP and extracted with 96% dioxane-water, other materials named in the same way