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A Method for Extracting High-Quality RNA from Diverse Plants for Next-Generation Sequencing and Gene Expression Analyses

✍ Scribed by Yockteng, Roxana; Almeida, Ana M. R.; Yee, Stephen; Andre, Thiago; Hill, Colin; Specht, Chelsea D.


Book ID
125332115
Publisher
Wiley (John Wiley & Sons)
Year
2013
Tongue
English
Weight
415 KB
Volume
1
Category
Article
ISSN
2168-0450

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## Abstract **BACKGROUND:** A fast and easy method to isolate RNA from grape (__Vitis vinifera__) berries is proposed. **RESULTS:** The procedure uses high concentrations of cetyltrimethylammonium bromide (CTAB) and includes boric acid with the purpose of increasing the RNA extraction. Eppendorf t