𝔖 Bobbio Scriptorium
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A method for extracting high-molecular-weight deoxyribonucleic acid from fungi

✍ Scribed by Charles A. Specht; Concetta C. DiRusso; Charles P. Novotny; Robert C. Ullrich

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
847 KB
Volume
119
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new method for isolating high-molecular-weight DNA from mycelium of the basidiomycete Schizophyllum commune is reported. Lyophilized mycelium is broken with mortar and pestle, and DNA extracted during gentle shaking in buffer containing 2% sodium dodecyl sulfate and toluene. DNA with double-strand length in excess of 80 kilobase pairs and singlestrand length of 36 kilobases can be prepared routinely in milligram quantities. This DNA is of high purity and suitable for reassociations and recombinant DNA studies. The procedure has also been used to prepare DNA from another filamentous basidiomycete, Heterobasidion annosum, and from yeast. With slight modification the procedure is attractive for isolating recombinant plasmids from yeast.

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