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A method for evaluating the use of fluorescent dyes to track proliferation in cell lines by dye dilution

✍ Scribed by Begum, Julfa; Day, William; Henderson, Carl; Purewal, Sukhveer; Cerveira, Joana; Summers, Huw; Rees, Paul; Davies, Derek; Filby, Andrew


Book ID
126625346
Publisher
John Wiley and Sons
Year
2013
Tongue
English
Weight
649 KB
Volume
83
Category
Article
ISSN
0196-4763

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A technique is described whereby the addition of low concentrations (millimolar to micromolar) of the fluorescent dye 1,8-ANS to the protein solution prior to crystallization results in crystallization experiments in which protein crystals are strongly contrasted above background artifacts when expo