A Method for Evaluating Phylogenetic Relationship of α-Satellite DNA Suprachromosomal Family by Nucleotide Frequency Calculation
✍ Scribed by Mikio Kato; Aya Kato; Nobuyoshi Shimizu
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 439 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1055-7903
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✦ Synopsis
The sequence similarity among chromosome-specific ␣-satellite DNA was quantitatively evaluated by a novel procedure: nucleotide frequency calculation. Tandemarrayed repetitive DNA segments were aligned with unit length repeat, and the nucleotide frequency at each position was used to estimate the phylogenetic distance between repetitive DNA segments. The calculations for human and chimpanzee X chromosome ␣-satellites showed that the results were consistent with the known relationships of primates, indicating that the nucleotide frequency calculation worked effectively to estimate the distances between satellite arrays. Human chromosome-specific ␣-satellites had been grouped into three suprachromosomal families (I, II, and III), and in the current work the nucleotide frequency analysis has defined the quantitative distances between the chromosome-specific ␣-satellite DNA.