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A method for eliminating Rayleigh scattering from fluorescence spectra

✍ Scribed by David I.C. Kells; Joe D.J. O'Neil; Theo Hofmann

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
221 KB
Volume
139
Category
Article
ISSN
0003-2697

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✦ Synopsis

A numerical method is described for the elimination of Rayleigh scattering from protein fluorescence emission spectra. The method is based upon the observation that Rayleigh scattering is symmetrical about a wavelength at or near the wavelength of excitation. It works best when an automated, computer-based approach can be applied to spectra collected on a data-logging device, although manual correction of chart-recorded spectra is also possible.

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