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A method for determining alkaline phosphatase activity in marine phytoplankton using spectrofluorometry

✍ Scribed by Melissa Blakely Peacock; Raphael M. Kudela


Book ID
116751291
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
310 KB
Volume
89
Category
Article
ISSN
0167-7012

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Alkaline phosphatase activity in subtrop
✍ M. J. Perry πŸ“‚ Article πŸ“… 1972 πŸ› Springer-Verlag 🌐 English βš– 725 KB

This paper describes a fluorometric method for assaying low levels of the enzyme alkaline phosphatase in seawater. The assay is based on the hydrolysis of the monophosphate ester bond of 3-0-methylfluorescein phosphate. This enzyme is synthesized by many microorganisms when phosphate becomes limitin